|
KMID : 0624620080410060466
|
|
BMB Reports
2008 Volume.41 No. 6 p.466 ~ p.471
|
|
|
Minimizing a QTL region for intramuscular fat content by characterizing the porcine Phosphodiesterase 4B (PDE4B) gene
|
|
Kim Jae-Hwan
Ovilo Cristina
Park Eung-Woo
Fernndez Almudena
Lee Jun-Heon
Jeon Jin-Tae
Lee Jung-Gyu
|
|
|
Abstract
|
|
|
|
Three isoforms of pig PDE4B were cloned and classified as two forms: PDE4B1 and PDE4B3, which contain UCR1 and UCR2; and PDE4B2, which contains only UCR2. The amino acid sequences of each isoform showed good conservation in human and rat. PDE4B2 is expressed in a wide range of tissues, but PDE4B1 and PDE4B3 are not. Using an informative SNP for the Iberian x Landrace intercross detected from intron 12, a linkage map was constructed. The location of PDE4B was estimated at 123.6 cM outside of the QTL-CI (124-128 cM) for IMF. However, the QTL-CI for IMF was reconfirmed with high significance, and its position was narrowed down to an interval of 4 cM (the region defined by markers PDE4B and SW1881). Using radiation hybrid mapping, LEPR, LEPROT, DNAJC6, AK3L1 and AK3L2 were selected as positional and/or functional candidates related to the QTL. [BMB reports 2008; 41(6): 466-471]
|
|
|
KEYWORD
|
|
|
Intramuscular fat, PDE4B, Positional candidate, Quantitative trait loci
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|